No2-3(2) 2019
Back to the issue
|
DOI 10.37219/2528-8253-2019-2-53 |
Melnikov O, Zabolotna D, Zayats T, Sydorenko T, Bredun O,
Prylutska A,
Kushnirenko T, Tymchenko M, Rylska O, Faraon I.
Immunomodulating properties of Cinnabsin |
Melnikov Oleg F.
State Institution “Institute of otolaryngology named after Prof. O.S.
Kolomiychenko of the National Academy of Medical Sciences of Ukraine”; Kyiv,
Ukraine.
Head of the Laboratory of Pathophysiology and Immunology (with a group of
pathomorphology)
Doctor of Medical Sciences, Professor
E-mail: elenamelnykova@gmail.com
Zabolotnaya Diana D
State Institution “Institute of otolaryngology named after Prof. O.S.
Kolomiychenko of the National Academy of Medical Sciences of Ukraine”; Kyiv,
Ukraine.
Clinical Allergology Department
Leading Researcher
Doctor of Medical Sciences
E-mail: dianazab@ukr.net
Zajac Tatyana A.
State Institution “Institute of otolaryngology named after Prof. O.S.
Kolomiychenko of the National Academy of Medical Sciences of Ukraine”; Kyiv,
Ukraine
Laboratory of Pathophysiology and Immunology (with a group of
pathomorphology)
Researcher
E-mail: amtc@kndio.kiev.ua
Sidorenko Tatyana V.
State Institution “Institute of otolaryngology named after Prof. O.S.
Kolomiychenko of the National Academy of Medical Sciences of Ukraine”; Kyiv,
Ukraine
Laboratory of Pathophysiology and Immunology (with a group of
pathomorphology)
Researcher
E-mail: amtc@kndio.kiev.ua
Bredun Alexander Yu
National Medical Academy of Post-Graduate Education Named After P.L. Shupik
Department of Pediatric Otorhinolaryngology, Audiology, Phoniatrics
Associate Professor, PhD.
E-mail: abredun@ukr.net
Kushnirenko Tetiana V.
Private Joint Stock Company "Naturfarm"
E-mail: tetiana.kushnirenko@alpenpharma.com
Timchenko Мarina D.
State Institution “Institute of otolaryngology named after Prof. O.S.
Kolomiychenko of the National Academy of Medical Sciences of Ukraine”; Kyiv,
Ukraine. Senior Researcher, Laboratory of Pathophysiology and Immunology
(with a group of pathomorphology), candidate of biological sciences.
E-mail: svtim@i.ua
Rylska Oksana G.
State Institution “Institute of otolaryngology named after Prof. O.S.
Kolomiychenko of the National Academy of Medical Sciences of Ukraine”; Kyiv,
Ukraine. Senior Researcher at the Department of Inflammatory Diseases of ENT
Organs, PhD.
Email: oxanar@ua.fm
Faraon Inna V
State Institution “Institute of otolaryngology named after prof. O.S.
Kolomiychenko of the National Academy of Medical Sciences of Ukraine”; Kyiv,
Ukraine
Laboratory of Pathophysiology and Immunology (with a group of
pathomorphology)
Junior Researcher
E-mail: amtc@kndio.kiev.ua |
|
Abstract
Introduction: Complex natural medicines are successfully used in
various inflammatory diseases of the upper respiratory tract, but their
immunomodulatory activity has not been studied enough, in particular in case
of Cinnabsin. Therefore, the aim of the study was to determine the effect of
the medicine on the production of cellular and humoral factors of immunity
and inflammation by tonsil cells of the children.
Materials and methods: The studies were performed in vitro using
24-hour cultures of children`s pharyngeal tonsil cells after adenotomy.
Altogether 90 samples of cultures were examined before and after the
influence of the medicine. The effect of complex natural medicine Cinnabsin
on the cells production of various cytokine groups (γ-interferon,
interleukins 1, 4, 10), expression of surface antigens CD14+, CD16+, and
cytolytic activity of natural killer cells were investigated. Methods of
enzyme immunoassay, light microscopy and cellular cytolysis with xenogenic
targets were used. Statistical processing was carried out with the Student`s
non-parametric criterion, and the Fisher`s angle transformation method when
processing ratios.
Results: Cinnabsin, both in dosage 10 μg/ml and 100μg/ml, increased
the number of cells with the expression of the antigen CD16+ and partially
CD14+, activated the natural cytotoxic cells of the tonsils, reduced the
production of IL-1β and increased the production of IL-10 and γ-interferon
in cell culture.
Conclusion: According to the sum of the effects and the
immunomodulation vectors, it is possible to state the modulating effect of
the medicine on the activity of various immunity reactions, which allows
concluding the pronounced anti-inflammatory and antiviral effect of
Cinnabsin. |
|
Keywords
Cinnabsin, tonsil cells culture, cytokines, cell cytolysis, CD-markers. |
|
Reference
- Gubler EB. [Mathematical methods of analysis and detection of
pathological processes]. Leningrad: Medicine; 1978. 294 p. [In Russian].
- Kovalchuk LV, Gankovskaya LV, Meshkova RY. [Clinical immunology and
allergology with the basics of general
immunology]. Moscow:
GEOTAR-Media; 2011. 640 p. [In Russian].
- Levitska SA. [The use of cinnabsin in the treatment and prevention of diseases of the upper respiratory tract in children]. Zhurnal ushnyh, nosovyh i
gorlovyh boleznej. 2018:(5с);62-5. [In Ukrainian].
- Melnikov PF, Rylskaya OG. Experimental study
of immunomodulatory properties of Tonsilgon H
in vitro. Zhurnal ushnyh, nosovyh i gorlovyh boleznej. 2005: (3); 74-6. [In Russian].
- Melnikov PF, Zayats TA. Comparative evaluation
of radioisotope and spectrophotometric methods
for recording cytolysis. Laboratory diagnostics.
1999: (2); 32-4. [In Russian].
- Novikov DK, Novikov PD. Method for determination of T and B lymphocytes by diagnosticums
based on monoclonal antibodies. Immunology.
2000: (2); 31-3. [In Russian].
- Yashan OI, Koritsky VG, Protasevich GS, Yashan KO, Chertkovsky RT. Adenoid vegetation
and adenoiditis. Zhurnal ushnyh, nosovyh i gorlovyh boleznej. 2013: (3с); 329-31. [In Ukrainian].
- Friese K, Zabolotny D. Homeopathie bei acute
rinosinusitis. HNO. 2007; 55: 271-7.
|
|
