No4-5(2) 2019
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DOI 10.37219/2528-8253-2019-4-13 |
Bredun O., Melnikov O., Tymchenko M.
Effects of plant-derived immunomodulators and lizak preparation in vitro
taken separately or in combination on the immune factors of palatine tonsils
of children with chronic tonsillitis
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Bredun Alexander Yu
National Medical Academy of Post-Graduate Education Named After P.L. Shupik
Department of Pediatric Otorhinolaryngology, Audiology, Phoniatrics
Associate Professor, PhD.
E-mail: abredun@ukr.net
ORCID ID: http://orcid.org/0000-0002-5013-7318
Researcher ID: X-2623-2018
Melnikov Oleg F.
State Institution “Institute of otolaryngology named after Prof. O.S.
Kolomiychenko of the National Academy of Medical Sciences of Ukraine”; Kyiv,
Ukraine.
Head of the Laboratory of Pathophysiology and Immunology (with a group of
pathomorphology)
Doctor of Medical Sciences, Professor
E-mail: elenamelnykova@gmail.com
ORCID ID: https://orcid.org/0000-0002-1261-8647
https://www.scopus.com/authid/detail.uri?authorId=7006385903
https://www.scopus.com/authid/detail.uri?authorId=7003452154
Timchenko Мarina D.
State Institution “Institute of otolaryngology named after Prof. O.S.
Kolomiychenko of the National Academy of Medical Sciences of Ukraine”; Kyiv,
Ukraine.
Senior Researcher
Laboratory of Pathophysiology and Immunology (with a group of
pathomorphology)
PhD
E-mail: svtim@i.ua |
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Abstract
Introduction: Chronic inflammatory diseases of the upper respiratory
tract of both microbial and viral origin occur due to
the immune deficiency of systemic and local nature. Among a large number of
immunomodulation
agents, a special role belongs to plant preparations, which are
characterized by relative harmlessness and high
efficiency, both in case of systemic and local use and they are often
combined with antimicrobial agents, therefore
the aim of the study was to investigate their effect on the reactions and
condition of lymphoid cells of tonsilswith
separate and combined use in vitro.
Methods: Cell suspensions were prepared mechanically and adjusted to a
concentration of 2 million/ml in
medium 199 with additives. Then, Lizak preparation was added to the cells
and starch solution was used in control.
Imupret and Esberitox preparations were added to the culture with or without
Lizak preparation. After cultivation,
the levels of proinflammatory cytokine – Interleukin-1β, pro-allergic factor
– Interleukin-4, Th-1 – derivative
of cytokine – interferon-γ were studied in the supernatant using the «Tsitokin
LLC» reagent kits (RF), as well
as tissue regeneration factor – transforming growth factor –TGF-1β
(Austria). The preparation was prepared from
the cell pellet, in which the relative nonviable cell count was determined
in a sample with trypan blue using a light
microscope (Olympus CX21FS1).The statistical analysis was performed using
Student's t-distribution.
Results: Plant-derived preparations did not have any effect on cell
viability in culture, did not reduce the
level of the pro-inflammatory cytokine interleukin-1β and did not stimulate
the production of the regeneration
factor TGF-1β. At the same time, the combined use of plant-derived
preparations and the antibacterial preparation
Lizak in cell culture of tonsils was accompanied by an increase in cell
viability compared to the use of Lizak
preparation separately, the stimulation of the regulatory antiviral factor
interferon-γ and an increase in the production
of tissue regeneration factor.
Conclusion: The immunomodulating properties of the antibacterial preparation
Lizak and plant-derived
immunomodulator Esberitox significantly differ in the points of application.
Both plant-derived preparations stimulated the production of γ-interferon by
tonsil cells in the presence of
Lizak preparation, the transforming growth factor TGF-1β, the viability of
tonsil cells was the highest with the
combination of Lizak and Esberitox. |
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Keywords
plant-derived preparations, antibacterial agents, tonsil cell cultures,
cytokines. |
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Reference
- Gubler EV. [Mathematical methods of the analysis and recognition of
pathological processes]. Moscow: Medicine; 1978. 294 p. [In Russian].
- Bredun O, Melnykov O. Immunological aspects in tht selection of
tonsils pathology treatment methods. The 8th International Symposium of
Tonsils.
Zurich; 2013. Abstr. 02.
- Drannik G. [Clinical immunology and allergology]. Kyiv:
Poligrafplius; 2010. 552 p. [In Russian].
- Ershov FI, Norovlianskii AN, Mezentseva MV. [Early cytokine
reactions in viral infections]. Cytokines and inflammation. 2004;3(1):3.
[In Russian].
- Kurchenko IF, Svidro SV, Savchenko VS. [The effect of Esberitox in
vitro on mononuclears of peripheral blood of patients with herpes virus
infection].
Immunology and allergology: Science and practice. 2016;1-4:19. [In
Ukrainian].
- Marushko Yu, Melnikov O, Movchan О, Lysovets O. Content of
antimicrobial peptides in oropharyngeal secretions of children suffering
from acute
respiratory diseases. Х Anniversary Ukrainian-Polish-Belorussian
Conference “Physiology and Pathology of Respiration: Advances in basic
research
and clinical applications” (Kyiv). Physiological journal. 2013;59(4):22.
- Мelnykov O, Zabolotny D. The concept of diagnosing secondary
immunodeficiency states based on determination of 16 immunoglobulins in
the secretions. International Journal on Immunorehabilitation.
2004;6(2):235.
- Melnykov OF. [Immunological aspects of the genesis of chronic
tonsillitis and regulation of functional activity of palatine tonsils].
[Dissertation].
Kyiv: Institute of Physiology of the Academy of Sciences of USSR; 1981.
294 s. [In Russian].
- Melnykov OF, Peleshenko NA, Zabolotnaiia DD, Rylskaia OG. [Immunomodulation
by plantderived preparations in the therapy of inflammatory
diseases of the upper respiratory tract]. Medical Nature. 2013:Sect. A:
108. [In Russian].
- Melnykov OF, Rylskaia OG, Tsymar AV, Peleshenko NA. [Imupret as
plant-derived immunomodulator or and adaptogen]. Medical Nature.
2013: Sect. B; 2(14):70. [In Russian].
- Melnykov OF, Zaiats TA, Tymchenko MD, Byl’ BN, Zemliak TB. [Study of
the effect of Lizak preparation on cellular and humoral immune factors
in vitro. Health of Ukraine. 2014;4:11. [In Russian].
- Melnykov OF, Zabolotnyi DI, Kishchuk VV, Bredun AYu, Rylskaia OG.
[Immunology of chronic tonsillitis]. Kyiv: Logos; 2017. 192 p. [In
Russian].
- Melnykov OF, Kryvokhatskaia LD, Levandovskaia VI, Faraon IV, Tsymar
AV. [Nonantibacterial effects in the mechanisms of action of local
antiseptics]. Otolaryngology. 2019;1-s:72. [In Russian].
- Yamanaka N. Moving towards a new era in the research of tonsils and
mucosal barriers. Adv Otorhinolaryngol. 2011;72:6-19.
doi:
10.1159/000324586.
- Tjabringa GS, Vos JB, Olthuis D, Ninaber DK, Rabe
KF. FEMS Immunol Med Microbiol. 2005 Aug
1;45(2):151-8.
doi: 10.1016/j.femsim.2005.03.004.
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