No4-5(2) 2019
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DOI 10.37219/2528-8253-2019-4-19 |
Bogomolov A.Ye., Dubchak L.I.
Comparison of diagnostic parameters of methods for determination of
sensitization to allergen D. pteronyssinus in patients with respiratory
allergic diseases
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Bogomolov Artemy Yevgenievich
Candidate of Medical Sciences, Associate Professor of the Department of
Phthisiatry with a course of clinical immunology and allergology of Vinnitsa
National Pirogov memorial Medical University
e-mail: art.bogomolov@gmail.com
https://orcid.org/0000-0002-5336-4858
Dubchak Lyudmila Ivanovna
Department of Phthisiatry with a course of clinical immunology and
allergology of Vinnitsa National Pirogov memorial Medical University,
assistant
email: doubchak_l@gmail.com
https://orcid.org/0000-0002-7438-8592 |
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Abstract
The aim of the study was to evaluate the diagnostic parameters of
various methods for determining allergy sensitization
to the dust mite allergen Dermatophagoides pteronyssinus in patients with
respiratory allergic diseases – allergic rhinitis and
bronchial asthma.
Materials and methods: During the study, 88 patients with allergic
rhinitis and / or atopic asthma were examined with
three different methods of specific allergic diagnosis (in vivo and in
vitro) according to the recommendations of the ethics
committee of the Pirogov Vinnitsa National Medical University, all of which
were out of acute period. Inclusion criteria were a
diagnosis of allergic rhinitis (both intermittent and persistent) and / or
atopic asthma. The prick-test was conducted according
to the classic test method by normative documents with commercial
allergen extracts. Western blots for the determination of IgE levels
were performed using RIDA AllergyScreen test systems
(R-Biopharm AG, Darmstadt, Germany) and Euroline (Euroimmun).
Results and discussion: The results of two systems for the
determination of specific IgE to D. Pteronyssinus by
the Rida AllergyScreen and Euroline methods have a systematic difference of
values (-1.27 kU / l).
There is a good agreement between the results of skin testing with D.
Pteronyssinus allergens and the detection of specific
IgE by the Rida AllergyScreen method, and there is a satisfactory agreement
between the results of skin testing with D.
Pteronyssinus allergens and detection of specific IgE by the Euroline
method.
According to the results of the curve of detection of specific IgE by
AllergyScreen method for determination of
sensitization to allergen D. Pteronyssinus has excellent diagnostic
significance (AUC=0,953), detection of specific IgE
by Euroline method for determination of sensitization to allergen D.
Pteronyssinus – good AUC=0,7. |
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Keywords
prick-test, allergy, immunoblotting, IgE. |
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