No6(2) 2019
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DOI 10.37219/2528-8253-2019-6-20 |
Bogomolov AYe
Comparative analysis of skin prick test with immunoblotting of different
manufacturers in the context of determining the sensitization to birch
allergen in patients with respiratory allergic diseases |
Bogomolov Artemii.Ye.
Associate Professor of the Department of Phthisiatry with a course of
clinical immunology and allergology of Vinnitsa National Pirogov memorial
Medical University
Candidate of Medical Sciences
Email: art.bogomolov@gmail.com
Orchid ID: https://orcid.org/0000-0002-5336-4858 |
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Abstract
The aim of the study was to evaluate the diagnostic parameters of
various methods for determining birch allergen sensitization in patients
with respiratory allergic diseases – allergic rhinitis and bronchial asthma.
Materials and methods: During the study, 88 patients with allergic
rhinitis and/or atopic asthma were examined with three different methods of
specific allergic diagnosis (in vivo and in vitro) according to the
recommendations of the ethics committee of the Pirogov Vinnitsa National
Medical University, all of which were out of acute. Inclusion criteria were
a diagnosis of allergic rhinitis (both intermittent and persistent) and/or
atopic asthma. The pre-test was conducted according to the classic test
method according to the normative documents with commercial allergen
extracts. Western blots for the determination of IgE levels were performed
using RIDA AllergyScreen test systems (R-Biopharm AG, Darmstadt, Germany)
and Euroline (Euroimmun).
Results and discussion: Systematic error of measurement results of
immunoblot of different manufacturers is equal to 2.27 ku/l, which indicates
the presence of a systematic difference. The distribution graph corresponds
to the type of absolute systematic error graphs. The standard deviation of
the difference was 11.28, which is significantly compared to the values
themselves.
Thus, the results of the two systems for the determination of specific IgE
for birch allergen by the Rida AllergyScreen and Euroline methods are not
well matched due to systematic divergence of indicators.
There is a good agreement between the results of skin testing with birch
allergens and the detection of specific IgE by the Rida AllergyScreen test,
and between the results of skin testing with birch allergen and the
detection of specific IgE by the Euroline method, there is a moderate
agreement between the results. |
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Keywords
pre-test, allergy, immunoblotting, IgE. |
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