No6(4) 2021
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DOI 10.37219/2528-8253-2021-6-70 |
Bondarchuk OD, Melnikov OF, Timchenko MD, Didyk ND
Experimental studies of humoral factors of inflammation and regeneration in
patients with injuries of the fronto-basal region |
Bondarchuk Oleksandr D.
Mykola Pyrohov Vinnytsia Regional Hospital, Vinnytsia, Ukraine
Department of Otolaryngology
PhD
E-mail: eskulap20009@gmail.com
ORCID ID: https://orcid.org/0000-0002-7636-9694
Melnikov Oleg F.
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of
National Academy of Medical Science of Ukraine”; Kyiv, Ukraine.
Head of the Laboratory of Pathophysiology and Immunology (with a group of
pathomorphology)
Doctor of Medical Sciences, Professor
E-mail: elenamelnykova@gmail.com
ORCID ID: https://orcid.org/0000-0002-1261-8647
https://www.scopus.com/authid/detail.uri?authorId=7006385903
https://www.scopus.com/authid/detail.uri?authorId=7003452154
Timchenko Мarina D.
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of
National Academy of Medical Science of Ukraine”; Kyiv, Ukraine.
Senior Researcher
Laboratory of Pathophysiology and Immunology (with a group of
pathomorphology)
PhD
E-mail: svtim@i.ua
ORCID ID: https://orcid.org/0000-0002-7889-1152
Didyk Natalia D.
Mykola Pyrohov Vinnytsia Regional Hospital (Vinnytsia, Ukraine)
Eye microsurgery department
Doctor microsurgery eye
E-mail: didyknd@gmail.com
ORCID ID: https://orcid.org/0000-0003-4313-1943 |
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Abstract
Materials and methods: Under the conditions of the experiment, the
authors conducted research of mononuclear blood
cultures were on the identification of сytokine factors of inflammation and
regeneration in patients with frontal bone trauma (29
patients) and practically healthy donors (11 persons). Factors of
inflammation (interleukin-1β, γ-interferon), anti-inflammation and
regeneration (interleukin-34 and transforming growth factor – TGF-1β) were
determined in the blood serum by ELISA, under the influence of medications
that remodulate bone matrix and regeneration – osteogenon and soluble
vitamin D3.
Results and conclusions: It was found that the osteogenon and
aquadetrim (vit. D3) have a positive influence on the production of
cytokines by blood mononuclears of the patients with FBT: they both decrease
the level of production of inflammatory cytokines in vitro and increase the
level of production of anti-inflammatory cytokines especially when used
together. The received data prove the expediency of including osteogenon and
aquadetrim in to the complex treatment of patients with frontal bone trauma. |
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Keywords
osteogenon, vitamin D3, frontobasal injury, interleukin-1β, interleukin 10,
γ-interferon, interleukin-4, transforming growth factor TGF-1β. |
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