SCIENTIFIC-PRACTICAL JOURNAL

| укр | рус |

 

 
Home Editorial ethics For authors Current issue Archive Contacts

No3-4(5) 2022

Back to the issue


DOI 10.37219/2528-8253-2022-3-36
Minin YuV, Virych PA, Karas AF, Chaika SP, Karas GA, Shuvalova NS, Kucherenko TI
Regeneration of artificial injuries external ear elastic cartilage of rabbits after stem cells local injection
Minin Yuriy V.
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Science of Ukraine”; Kyiv, Ukraine.
Department of inflammatory diseases of ENT-organs, leading researcher.
Doctor of Medical Sciences, Professor
ORCID ID: 0000-0002-7836-3480
Scopus Author ID: 6508004691
Email: mininy1956@gmail.com

Virych Pavlo A.
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Science of Ukraine”; Kyiv, Ukraine
Laboratory of Biophysics
Researcher, PhD of Biological Sciences
E-mail: sphaenodon@ukr.net
ORCID ID: https://orcid.org/0000-0002-1463-1992
Scopus Author ID: 57193401578

Karas Anton F.
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of
National Academy of Medical Science of Ukraine”; Kyiv, Ukraine
Laboratory of Biophysics
Head of Laboratory, PhD of Biological Sciences
E-mail: akaras2022@gmail.com
ORCID ID: https://orcid.org/0000-0003-2324-780X

Chaika Svitlana P
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Science of Ukraine”; Kyiv, Ukraine
Laboratory of Biophysics
Senior researcher
Candidate of Biological Sciences
E-mail: svetlach555@gmail.com
ORCID ID: https://orcid.org/0000-0003-4324-5529

Karas Halyna A.
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of
National Academy of Medical Science of Ukraine”; Kyiv, Ukraine
Laboratory of Biophysics,
Leading researcher, PhD of Biological Sciences
E-mail: gkaras@ukr.net
ORCID ID: https://orcid.org/0000-0003-3098-3813

Shuvalova Nagiya S
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of
National Academy of Medical Science of Ukraine”; Kyiv, Ukraine
Laboratory of Biophysics
Senior Research Fellow, PhD of Medical Sciences
E-mail: Shuvalovanadiia@gmail.com
ORCID ID: https://orcid.org/0000-0002-6390-5996

Kucherenko Tetiana I.
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Science of Ukraine”; Kyiv, Ukraine.
Department of inflammatory diseases of ENT-organs, Senior Researcher
E-mail: tkucherenko@hotmail.com
ORCID ID: https://orcid.org/0000-0002-2158-8101
Scopus Author ID: 6701383048

Abstract

Introduction: Every year, a large number of patients from developed countries turn to surgical departments with the reconstruction problems of the auricle cartilage. A some of surgical procedures was developed to correct minor defects due to the low regenerative capacity of elastic cartilage. Stem cells can potentially differentiate into chondroblasts and chondrocytes and restore cartilage integrity. Many factors influence on the differentiation and proliferation of stem cells, which complicates the method application. Therefore, the investigation of using stem cells to regeneration elastic cartilage is relevant.
Aim: clarification the regeneration features of the artificial defect of the rabbits’ ear elastic cartilage after the stem cells injection.
Materials and methods: The investigation was conducted on rabbits of chinchilla breed aged 1.5 months, weighing 2.5 kg. Artificial lesions measuring 2 x 7-10 mm were simulated on the cartilaginous plate of the outer ear with a scalpel. 0.5 ml of stem cell suspension (~5 million) obtained from the umbilical cord of rabbits by enzymatic method was injected into the defect site. Histological sections of the cartilage defect were prepared. The samples were stained by Weigert and Azure II methods. The relative density of collagen, elastin fibers, oxyphilic and basophilic tissue elements was programmatically evaluated.
Results and discussion: The amount of fibers in the native elastic cartilage was more on 35% of the relative dermis. The newly formed tissues at the damage cartilage was like to the dermis after 2 months. Similar results were found on ear in 2.5 and 3 months after surgery. Native cartilage on histological sections almost was not oxyphilic. Maybe a significant number of acidic components was masked it. Dense connective tissue contains almost equal amounts of both components. Thus, it is possible to trace changes in the intercellular matrix that will characterize the direction of the regeneration process. The area of elastic cartilage defect was characterized by a significant presence of oxyphilic elements after 2 months of the stem cells addition. The ratio of oxyphilic and basophilic components in the native elastic cartilage was about to zero. For the dermis was 1.4-1.5. Dense scar connective tissue was characterized by a ratio about 1. Based on the results obtained, was assumed that the direction of stem cell proliferation is determined shortly after injection.
Conclusion: The addition of stem cells to the area of the artificially created elastic cartilage defect of the rabbit's ear was not allow to obtain stable regenerative processes of cartilage tissue and the restoration of the intercellular matrix to its original state.
The process of stem cell differentiation contributes the formation of dense connective tissue of the scar, which may be due to the presence of proinflammatory cytokines, growth factors and other biologically active molecules. This was not creating the necessary conditions for the cartilage regeneration.

Keywords

stem cells, elastic cartilage, regeneration.


Reference

  1. Brianezi G, Grandi F, Bagatin E, Enokihara MM, Miot HA. Dermal type I collagen assessment by digital image analysis. An Bras Dermatol. 2015; 90(5):723-7. doi:10.1590/abd1806-4841.20153331
  2. Chang SC, Tobias G, Roy AK, Vacanti CA, Bonassar LJ. Tissue engineering of autologous cartilage for craniofacial reconstruction by injection molding. Plast Reconstr Surg. 2003;112(3):793-801. doi: 10.1097/01.PRS.0000069711.31021.94
  3. Chen C, Huang K, Zhu J, Bi Y, Wang L, Jiang J, Zhu T, Yan X, Zhao J. A novel elastic and controlled-release poly(ether-ester-urethane)urea scaffold for cartilage regeneration. J Mater Chem B. 2020;18(8):4106-21. doi:10.1039/c9tb02754h.
  4. Ciorba A, Martini A. Tissue engineering and cartilage regeneration for auricular reconstruction. Int J Pediatr Otorhinolaryngol. 2006;70(9):1507-15. doi:10.1016/j.ijporl.2006.03.013.
  5. de Chalain T, Phillips JH, Hinek A. Bioengineering of elastic cartilage with aggregated porcine and human auricular chondrocytes and hydrogels containing alginate, collagen, and kappa-elastin. J Biomed Mater Res. 1999;44(3):280-8. doi: 10.1002/(sici)1097-4636(19990305)44:3<280::aidjbm6>3.0.co;2-h.
  6. Ebrahimi A, Ahmadi H, Pourfraidon Ghasrodashti Z, Tanide N, Shahriarirad R, Erfani A, Ranjbar K, Ashkani-Esfahani S. Therapeutic effects of stem
    cells in different body systems, a novel method that is yet to gain trust: A comprehensive review. Bosn J Basic Med Sci. 2021;21(6):672-701. doi: 10.17305/bjbms.2021.5508.
  7. Fosang AJ, Beier F. Emerging Frontiers in cartilage and chondrocyte biology. Best Pract Res Clin Rheumatol. 2011;25(6):751-66. doi: 10.1016/j.berh.2011.11.010.
  8. Garcнa-Lуpez J, Garciadiego-Cбzares D, Melgarejo-Ramнrez Y, Sбnchez-Sбnchez R, Solнs-Arrieta L, Garcнa-Carvajal Z, Sбnchez-Betancourt JI, Ibarra C, Luna-Bбrcenas G, Velasquillo C. Chondrocyte differentiation for auricular cartilage reconstruction using a chitosan based hydrogel. Histol Histopathol. 2015;30(12):1477-85. doi: 10.14670/HH-11-642.
  9. Garcнa-Martнnez L, Campos F, Godoy-Guzmбn C, Del Carmen Sбnchez-Quevedo M, Garzуn I, Alaminos M, Campos A, Carriel V. Encapsulation of human elastic cartilage-derived chondrocytes in nanostructured fibrin-agarose hydrogels. Histochem Cell Biol. 2017;147(1):83-95. doi: 10.1007/s00418-016-1485-9.
  10. Grubbs H, Manna B. Wound Physiology. 2021. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2022.
  11. Hou M, Tian B, Bai B, Ci Z, Liu Y, Zhang Y, Zhou G, Cao Y. Dominant role of in situ native cartilage niche for determining the cartilage type regenerated by BMSCs. Bioact Mater. 2021; 13:149-160. doi: 10.1016/j.bioactmat.2021.11.007.
  12. Jayasuriya CT, Chen Y, Liu W, Chen Q. The influence of tissue microenvironment on stem cellbased cartilage repair. Ann N Y Acad Sci. 2016; 1383(1):21-33. doi: 10.1111/nyas.13170.
  13. Jessop ZM, Javed M, Otto IA, Combellack EJ, Morgan S, Breugem CC, Archer CW, Khan IM, Lineaweaver WC, Kon M, Malda J, Whitaker IS. Combining regenerative medicine strategies to provide durable reconstructive options: auricular cartilage tissue engineering. Stem Cell Res Ther. 2016;7:19. doi: 10.1186/s13287-015-0273-0.
  14. Khan S, Almйciga-Dнaz CJ, Sawamoto K, Mackenzie WG, Theroux MC, Pizarro C, Mason RW, Orii T, Tomatsu S. Mucopolysaccharidosis IVA and glycosaminoglycans. Mol Genet Metab. 2017; 120(1-2):78-95. doi: 10.1016/j.ymgme.2016.11.007.
  15. Kobayashi S, Takebe T, Inui M, Iwai S, Kan H, Zheng YW, Maegawa J, Taniguchi H. Reconstruction of human elastic cartilage by a CD44+ CD90+ stem cell in the ear perichondrium. Proc Natl Acad Sci USA. 2011;108(35):14479-84. doi: 10.1073/pnas.1109767108.
  16. Li L, Yu F, Zheng L, Wang R, Yan W, Wang Z, Xu J, Wu J, Shi D, Zhu L, Wang X, Jiang Q. Natural hydrogels for cartilage regeneration: Modification, preparation and application. J Orthop Translat. 2018;17:26-41. doi: 10.1016/j.jot.2018.09. 003.
  17. Min SH, Kim JH, Lee MI, Kwak HH, Woo HM, Shim JH, Choi DM, Lee JS, Jeong JY, Kang BJ. Evaluation of Auricular Cartilage Reconstruction Using a 3-Dimensional Printed Biodegradable Scaffold and Autogenous Minced Auricular Cartilage. Ann Plast Surg. 2020;85(2):185-193. doi: 10.1097/SAP.0000000000002313.
  18. Miyanaga T, Ueda Y, Miyanaga A, Yagishita M, Hama N. Angiogenesis after administration of  basic fibroblast growth factor induces proliferation and differentiation of mesenchymal stem cells in elastic perichondrium in an in vivo model: mini review of three sequential republication-abridged reports. Cell Mol Biol Lett. 2018;23:49. doi: 10.1186/s11658-018-0113-1.
  19. Rastogi P, Kandasubramanian B. Review of alginate-based hydrogel bioprinting for application in tissue engineering. Biofabrication. 2019;11(4): 042001. doi: 10.1088/1758-5090/ab331e.
  20. Stephan S, Reinisch J. Auricular Reconstruction Using Porous Polyethylene Implant Technique. Facial Plast Surg Clin North Am. 2018;26(1):69-85. doi: 10.1016/j.fsc.2017.09.009.
  21. Vynios DH. Metabolism of cartilage proteoglycans in health and disease. Biomed Res Int. 2014;2014:452315. doi: 10.1155/2014/452315.
 

© 2019, Public Organization «Ukrainian Scientific Medical Society of Otorhinolaryngologists»