SCIENTIFIC-PRACTICAL JOURNAL

| укр | рус |

 

 
Home Editorial ethics For authors Current issue Archive Contacts

No5(5) 2022

Back to the issue


DOI 10.37219/2528-8253-2022-5-46
Deryabina O, Minin Y, Karas G, Kucherenko T, Maslova O, Shuvalova N, Deriabin O,
Tarasov O, Minina A, Kordium V
Human umbilical cord-derived mesenchymal stem cells promote regeneration of nasal mucosa atrophy      
Deryabina Olena
State Institution «Academician M.D. Strazhesko National Scientific Center «Institute of Cardiology, Clinical and Regenerative Medicine» of the National Academy of Medical Sciences of Ukraine», Kyiv, Ukraine
Senior Researcher
PhD
ORCID ID: http://orcid.org/0000-0002-2934-5171

Minin Yuriy V.
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Science of Ukraine”; Kyiv, Ukraine.
Department of inflamma to rydiseasesof ENT-organs, leading researcher.
Doctor of Medical Sciences, Professor
ORCID ID: 0000-0002-7836-3480
ScopusAuthor ID: 6508004691
Email: mininy1956@gmail.com

Karas Halyna A.
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of
National Academy of Medical Science of Ukraine”; Kyiv, Ukraine
Laboratory o fBiophysics,
Leading researcher, Ph D of Biological Sciences
E-mail: gkaras@ukr.net
ORCID ID: https://orcid.org/0000-0003-3098-3813

Kucherenko Tetiana I.
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Science of Ukraine”; Kyiv, Ukraine.
Department of inflamma to rydiseases of ENT-organs, SeniorResearcher
E-mail: tkucherenko@hotmail.com
ORCID ID: https://orcid.org/0000-0002-2158-8101
ScopusAuthor ID: 6701383048

Maslova Olga
State Institution «Academician M.D. Strazhesko National Scientific Center «Institute of Cardiology, Clinical and Regenerative Medicine» of the National Academy of Medical Sciences of Ukraine», Kyiv, Ukraine
Senior Researcher
https://orcid.org/0000-0002-9372-896X

Shuvalova Nadiya
State Institution«O.S. Kolomiychenko Institute of Otolaryngology ofNational Academy of Medical Science of Ukraine»; Kyiv, Ukraine
Laboratory of Biophysics
Senior Research Fellow, Ph D of Medical Sciences
E-mail: Shuvalovanadiia@gmail.com
ORCIDID: https://orcid.org/0000-0002-6390-5996

Deriabin Oleg
State Scientific Control Institute of Biotechnology and Strains of Microorganisms (SSCIBSM)
Head of the Department of Molecular Biology
E-mail: admin@biocontrol.com.ua
https://orcid.org/0000-0001-5702-0190

Tarasov Oleksandr
State Institution «Academician M.D. Strazhesko National Scientific Center «Institute of Cardiology, Clinical and Regenerative Medicine» of the National Academy of Medical Sciences of Ukraine», Kyiv, Ukraine
https://orcid.org/0000-0003-1481-5529

Minina Ganna
State Institution “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Science of Ukraine”; Kyiv, Ukraine.
Ear Microsurgery and Otoneurosurgery Department
Researcher
Candidate of Medical Sciences
Email: mininy1956@gmail.com
https://orcid.org/0000-0003-1237-4395

Kordium Vitalii
State Institution «Academician M.D. Strazhesko National Scientific Center «Institute of Cardiology, Clinical and Regenerative Medicine» of the National Academy of Medical Sciences of Ukraine», Kyiv, Ukraine
https://orcid.org/000-0002-1324-2231

Abstract

Introduction: Mesenchymal multipotent stromal cells (mesenchymal stem cells-MSCs) are currently the most promising and widely used means of cell therapy.
Common sources for obtaining them are bone marrow and adipose tissue, but now the umbilical cord and placenta are gaining more and more popularity, since the cells isolated from them have a number of advantages over other sources.
Aim: To study the peculiarities of human umbilical cord MSCs influence of on the regeneration of the mucous membrane of the nasal cavity in experimentally induced atrophy.
Materials and methods: 30 laboratory mice were observed, which divided into two experimental groups (10 animals each) and control group (10 mice). Clinical and morphological studies were perfoemed 1 and 2 months after the development of atrophic rhinitis.
Results: Umbilical cords were obtained after timely delivery, chopped into small fragments and cultured in the appropriate nutrient medium with all supplements known for MSCs. MSCs migrated from the pieces, formed colonies, and after the formation of a 70-80% confluent layer, they were detached from the surface in the usual way and transferred to new vials. This procedure was performed twice, after which the cells were characterized by surface markers (positive and negative for MSCs) and used for administration to model animals. Atrophic rhinitis in mice was developed using 3 pathogenic strains of Pasteruella and confirmed by clinical and morphological characteristics. MSCs (characterized, at the 2nd passage) were injected intravenously (1×106 per mouse) and experimental animals were observed for 2 months. The clinical condition of the animals was examined once a week.
After the end of the experiment, a postmortem morphological study was performed. Clinical and morphological data showed positive effect of transplantation of human umbilical cord MSCs for nasal mucosa regeneration in mice compared to untreated controls.

Keywords

Mesenchymal stromal cells, multipotent stromal cells, human umbilical cord, atrophic rhinitis, mucous membrane of the nasal cavity.


Reference

  1. Spencer ND, Gimble JM, Lopez MJ. Mesenchymal stromal cells: past, present, and future. Vet Surg. 2011;40(2):129-39. doi: 10.1111/j.1532-950 X.2010.00776.x.
  2. Salem HK, Thiemermann C. Mesenchymal stromal cells: current understanding and clinical status. Stem Cells. 2010;28(3):585-96. doi: 10.1002/stem.269.
  3. Bianco P, Robey PG, Simmons PJ. Mesenchymal stem cells: revisiting history, concepts, and assays. Cell Stem Cell. 2008;2(4):313-9. doi: 10.1016/j.stem.2008.03.002.
  4. Ciccocioppo R, Cangemi GC, Roselli EA, Kruzliak P. Are stem cells a potential therapeutic tool in coeliac disease? Cell Mol Life Sci. 2015; 72(7):1317-29. doi: 10.1007/s00018-014-1797-7.
  5. Ciccocioppo R, Gallia A, Sgarella A, Kruzliak P, Gobbi PG, Corazza GR. Long-term follow-up of Crohn disease fistulas after local injections of bone marrow-derived mesenchymal stem cells. Mayo Clin Proc. 2015;90(6):747-55. doi: 10.1016/j.
    mayocp.2015.03.023.
  6. Fu X, Liu G, Halim A, Ju Y, Luo Q, Song G. Mesenchymal stem cell migration and tissue repair. Cells. 2019;8(8):784. doi:10.3390/cells8080784.
  7. Hu C, Li L. Preconditioning influences mesenchymal stem cell properties in vitro and in vivo. J Cell Mol Med. 2018;22(3):1428-42. doi: 10.1111/ jcmm. 13492.
  8. Liu S, Liu F, Zhou Y, Jin B, Sun Q, Guo S. Immunosuppressive Property of MSCs Mediated by Cell Surface Receptors. Front Immunol. 2020;11:1076. doi: 10.3389/fimmu.2020.01076.
  9. Islam A, Urbarova I, Bruun JA, MartinezZubiaurre I. Large-Scale secretome analyses unveil the superior immunosuppressive phenotype of umbilical cord stromal cells compared to other adult mesenchymal stromal cells. European Cells and Materials. 2019; 37:153-74.
  10. Dabrowski FA, Burdzinska A, Kulesza A, Sladowska A, Zolocinska A, Gala K, Paczek L, Wielgos M. Comparison of the paracrine activity of mesenchymal stem cells derived from human umbilical cord, amniotic membrane and adipose tissue. J Obstet Gynaecol Res. 2017;43(11):1758-68. doi: 10.1111/jog.13432
  11. Gnecchi M, Danieli P, Malpasso G, Ciuffreda MC. Paracrine mechanisms of mesenchymal stem cells in tissue repair. Methods Mol Biol. 2016;1416: 123-46. doi: 10.1007/978-1-4939-3584-0_7.
  12. Friedenstein AJ, Chailakhjan RK, Lalykina KS. The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Tissue Kinet. 1970;3(4):393-403. doi: 10.1111/j.1365-2184.1970.tb00347.x.
  13. Dietz AB, Padley DJ, Butler GW, Anderson JM, Sarr MG, Windebank AJ, Textor SC, Kudva YC, Galanis E, Peng K, Gastineau DA. Data in support of the clinical use of adipose derived MSC: growth, storage, function and safety. Cytotherapy. 2013; 15: S5.
  14. Sánchez PL, Sanz-Ruiz R, Fernández-Santos ME, Fernández-Avilés F. Cultured and freshly isolated adipose t issue-derived cells: fat years for cardiac stem cell therapy. Eur Heart J. 2010;31(4):394-7. doi: 10.1093/eurheartj/ehp403.
  15. de Girolamo L, Niada S, Arrigoni E, Di Giancamillo A, Domeneghini C, Dadsetan M, Yaszemski MJ, Gastaldi D, Vena P, Taffetani M, Zerbi A, Sansone V, Peretti GM, Brini AT. Repair of osteochondral defects in the minipig model by OPF hydrogel loaded with adipose-derived mesen chymal stem cells. Regen Med. 2015;10(2):135-51. doi: 10.2217/rme.14.77.
  16. Zack-Williams SD, Butler PE, Kalaskar DM. Current Progress in Use of Adipose Derived Stem Cells in Peripheral Nerve Regeneration. World J Stem Cells. 2015;7(1):51-64. doi: 10.4252/wjsc.v7.i1.51.
  17. Williams JT, Southerland SS, Souza J, Calcutt AF, Cartledge RG. Cells isolated from adult human skeletal muscle capable of differentiating into multiple mesodermal phenotypes. Am Surg. 1999; 65(1):22-6.
  18. Kassis I, Zangi L, Rivkin R, Levdansky L, Samuel S, Marx G, Gorodetsky R. Isolation of mesenchymal stem cells from GCSF-mobilized human peripheral blood using fibrin micro-beads. Bone Marrow Transplant. 2006;37(10):967-76. doi: 10.1038/sj.bmt.1705358.
  19. Hennrick KT, Keeton AG, Nanua S, Kijek TG, Goldsmith AM, Sajjan US, Bentley JK, Lama VN, Moore BB, Schumacher RE, Thannickal VJ, Hershenson MB. Lung cells from neonates show a mesenchymal stem cell phenotype. Am J Respir Crit Care Med. 2007;175(11):1158-64. doi: 10.1164/rccm.200607-941OC.
  20. Chong JJ, Chandrakanthan V, Xaymardan M, Asli NS, Li J, Ahmed I, Heffernan C, Menon MK, Scarlett CJ, Rashidianfar A, Biben C, Zoellner H, Colvin EK, Pimanda JE, Biankin AV, Zhou B, Pu WT, Prall OW, Harvey RP. Adult cardiac-resident MSC-like stem cells with a proepicardialorigin. Cell Stem Cell. 2011;9(6):527-40. doi: 10.1016/j.stem.2011.10.002.
  21. Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA. 2000; 97(25):13625-30. doi: 10.1073/pnas.240309797.
  22. Cho NH, Park YK, Kim YT, Yang H, Kim SK. Lifetime expression of stem cell markers in the uterine endometrium. Fertil Steril. 2004;81(2):403-7. doi: 10.1016/j.fertnstert.2003.07.015.
  23. Fukuchi Y, Nakajima H, Sugiyama D, Hirose I, Kitamura T, Tsuji K. Human placenta-derived cells have mesenchymal stem/progenitor cell potential. Stem Cells. 2004;22(5):649-58. doi: 10.1634/stemcells.22-5-649.
  24. Alviano F, Fossati V, Marchionni C, Arpinati M, Bonsi L, Franchina M, Lanzoni G, Cantoni S, Cavallini C, Bianchi F, Tazzari PL, Pasquinelli G, Foroni L, Ventura C, Grossi A, Bagnara GP. Term amniotic membrane is a high throughput source for
    multipotent mesenchymal stem cells with the ability to differentiate into endothelial cells in vitro. BMC Dev Biol. 2007;7:11. doi: 10.1186/1471-213X-7-11.
  25. Wang HS, Hung SC, Peng ST, Huang CC, Wei HM, Guo YJ, Fu YS, Lai MC, Chen CC. Mesenchymal stem cells in the Wharton’s jelly of the human umbilical cord. Stem Cells. 2004;22(7):1330-7. doi: 10.1634/stemcells.2004-0013.
  26. Taghizadeh RR, Cetrulo KJ, Cetrulo CL. Wharton’s Jelly stem cells: Future clinical applications. Placenta. 2011;32 Suppl 4:S311-5. doi: 10.1016/j.placenta.2011.06.010.
  27. Fong CY, Chak LL, Biswas A, Tan JH, Gauthaman K, Chan WK, Bongso A. Human Wharton’s jelly stem cells have unique transcriptome profiles compared to human embryonic stem cells and other mesenchymal stem cells. Stem Cell Rev
    Rep. 2011;7(1):1-16. doi: 10.1007/s12015-010-9166-x.
  28. Anzalone R, Lo Iacono M, Corrao S, Magno F, Loria T, Cappello F, Zummo G, Farina F, La Rocca G. New emerging potentials for human Wharton’s jelly mesenchymal stem cells: immunological features and hepatocyte-like differentiative ca
    pacity. Stem Cells Dev. 2010;19(4):423-38. doi: 10. 1089/scd.2009.0299.
  29. Maslova OA, Kordium VA, Deryabina OG, Eds De Bartolo L, Bader A. Umbilical cord matrix cells: promising instrument for regenerative medicine. Biomaterials for Stem Cell Therapy: State of the Art and Vision for the Future New York: CRC Press. 2013.
  30. Bieback K, Brinkmann I. Mesenchymal stromal cells from human perinatal t issues: from biology to cell therapy. World J Stem Cells. 2010;2(4):81-92. doi: 10.4252/wjsc.v2.i4.81.
  31. Nekanti U, Mohanty L, Venugopal P, Balasubramanian S, Totey S, Ta M. Optimization and scaleup of Wharton’s jelly-derived mesenchymal stem cells for clinical applications. Stem Cell Res. 2010; 5(3): 244-54. doi: 10.1016/j.scr.2010.08.005.
  32. Tsagias N, Koliakos I, Karagiannis V, Eleftheriadou M, Koliakos GG. Isolation of mesenchymal stem cells using the total length of umbilical cord for transplantation purposes. Transfus Med. 2011; 21(4):253-61. doi: 10.1111/j.1365-148.2011.
    01076.x.
  33. De Bruyn C, Najar M, Raicevic G, Meuleman N, Pieters K, Stamatopoulos B, Delforge A, Bron D, Lagneaux L. A rapid, simple, and reproducible method for the isolation of mesenchymal stromal cells from Wharton’s jelly without enzymatic treatment. Stem Cells Dev. 2011;20(3):547-57. doi: 10.1089/scd.2010.0260.
  34. Tong CK, Vellasamy S, Tan BC, Abdullah M, Vidyadaran S, Seow HF, Ramasamy R. Generation of mesenchymal stem cell from human umbilical cord tissue using a combination enzymatic and mechanical disassociat ion method. Cell Biol Int. 2011;35(3):221-6. doi: 10.1042/CBI20100326.
  35. Can A, Karahuseyinoglu S. Concise review: human umbilical cord stroma with regard to the source of fetus-derived stem cells. Stem Cells. 2007; 25(11):2886-95. doi: 10.1634/stemcells.2007-0417.
  36. Hendijani F, Sadeghi-Aliabadi H, Javanmard SH. Comparison of human mesenchymal stem cells isolated by explant culture method from entire umbilical cord and Wharton’s jelly matrix. Cell Tissue Bank. 2014;15(4):555-65. doi: 10.1007/s10561-014-9425-1.
  37. Maslova ОО, Shuvalova NS, Sukhorada OM, Deryabina OG, Kordium VA, inventors; State Institute of Genetic and Regenerative Medicine NAMS of Ukraine, assignee. Method of obtaining MSC from human umbilical cord. Ukrainian patent
    UA 74171, 2012 Oct 25.
  38. Dutt SN, Kameswaran M. The aetiology and management of atrophic rhinitis. J Laryngol Otol. 2005; 119(11):843-52. doi: 10.1258/002221505774783377.
  39. Maslova O, Novak M, Kruzliak P. Umbilical Cord Tissue-Derived Cells as Therapeutic Agents. Stem Cells Int. 2015;2015:150609. doi: 10.1155/2015/150609.
  40. Kozlovsky LV, Nikolaev AY. [Textbook of clinical laboratory studies]. Moscow: Medicine; 1984. pp. 288. [In Russian].
  41. Hawley TS, Hawley RG. Flow Cytometry Protocols. Second edition. Edited by Hawley TS and Hawley RG. Series: Methods in Molecular Biology, №263. Totowa, NJ: Humana Press Inc. 2004. pp. 424.
  42. Jordan RW, Roe JM. An experimental mouse model of progressive atrophic rhinitis of swine. Vet Microbiol. 2004;103(3-4):201-7. doi: 10.1016/j.vetmic. 2004.07.006.
  43. Mіnіn YV, Karas AF, Kucherenko TІ, Karas GA, Tarasov OA. [Infective genesis atrophic rhinitis experimental model]. Rinologіya. 2012;4:40-5. Available from: http://www.lorlife.kiev.ua/ rhinology/ 2012/2012_4_40.pdf. [Article in Ukrainian].
  44. Dominici M, Le Blanc K, Mueller I, SlaperCortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop DJ, Horwitz E. Minimal criteria for defining multipotent mesenchymal stromal cells. Cytotherapy. 2006;8(4):315-7. doi: 10.1080/14653240600855905.
  45. Khubutiya MS, Vagabov AV, Temnov AA, Sklifas AN. Paracrine mechanisms of proliferative, antiapoptotic and antiinflammatory effects of mesenchymal stromal cells in models of acute organ injury. Cytotherapy. 2014 May;16(5):579-85. doi: 10.1016/j.jcyt.2013.07.017.
 

© 2019, Public Organization «Ukrainian Scientific Medical Society of Otorhinolaryngologists»