SCIENTIFIC-PRACTICAL JOURNAL

| укр | рус |

 

 
Home Editorial ethics For authors Current issue Archive Contacts

No1(4) 2021

Back to the issue


DOI 10.37219/2528-8253-2021-1-46
Shkorbotun YaV, Skoryk MA
Peculiarities of the chemical composition of fungal bodies of the paranasal sinuses
Shkorbotun Yaroslav V.
State Institution of Science "Research and Practical Center of Preventive and Clinical Medicine" State Administrative Department, Department of miniinvasive surgery, Kyiv, Ukraine
Shupyk National Medical Academy of Postgraduate Education, Department of Otolaryngology, Kyiv, Ukraine
Candidate of Medical Sciences, Associate Professor
Email: lorkiev@ukr.net
Orchid ID: http://orcid.org/0000-0002-3103-7194

Skoryk Mykola A.
Metallophysics Institute of the National Academy of Sciences of Ukraine, Kyiv
Head of the Laboratory of Electron Microscopy at the Metallophysics Institute of the National Academy of Sciences of Ukraine, Kyiv
Email: mykolaskor@gmail.com

Abstract

Introduction: The significance of fungal microflora in the development of chronic rhinosinusitis tends to increase.
The purpose of the study: We had studied the chemical composition of fungal bodies of odontogenic and nonodontogenic origin to determine the relationship between fungal sinusitis and previous endodontic treatment.
Methods and materials: The 7 samples of the most common dental endodontic materials and 32 fungal ball removed from the paranasal sinuses in patients were examined by electron microscopy and masspectrometry: 22 of them were from the maxillary sinus in persons with a probable odontogenic cause of the disease (group 1), and 10 with the exception of odontogenic factor – 5 from the maxillary sinus - group 2 and 5 - from the sphenoid - group 3).
The resultsand disscusion: In areas with high radiation density of fungal balls, its level in group 1 was 1173.6 ± 257.9 HU, groups 2 and 3 - 283.2 ± 21.8 HU and 262.6 ± 3 HU, and the radiation density from the samples with base it’s level in all groups did not differ among them and was from 50.1 to 55.0 HU.
It was found that in all samples dental materials there was a content of Zn with a mass fraction from 0.71 ± 0.42% to 85.51 ± 0.05%. When analyzing the structure of fragments of fungal balls from areas with a base level of radiation density in patients of all three groups, its homogeneity and absence of crystalline inclusions were noted.
In contrast, in fragments of fungal ball from areas of high radiation density there where a significant number of crystals in individuals with established odontogenic factor (group 1) and their absence in the material of groups 2 and 3. In the composition of samples of fungal balls from areas with high radiation density, it was found that in group №1 in 10 samples (45.45%) showed a significant content of Zinc (average mass fraction - 23.71 ± 4.45%), and Calcium was found in 40.9% of the samples at the level of 3.8 ± 1.33%.
The chemical composition of areas of fungal bodies with high radiation density in individuals with excluded odontogenic factor (group 2 and group 3) was similar and was characterized by the presence of high level of Calcium from areas with increased radiation density of 24.38 ± 6.22%, and 22.04 ± 5,72%, while the background (group 2 - 1.26 ± 0.33%, group 3 –0.90 ± 0.34%). Zinc and other chemical elements - markers of dental materials in groups 2 and 3 were not detected.
Conclusions: All dental materials we examined contain Zn and chemical elements are not characteristic of tissues of biological origin (Ba, Zr, W, Co and Ti), the content of which may cause increased radiological density of the tissue of the fungal ball.
Elevated Zn and Ca content was detected in 45.45% and 40.9% of samples of fungal ball of odontogenic origin, and in almost all samples of fungal ball of neodontogenic origin, regardless of the location of the sinus (maxillary or sphenoidal), Ca was detected, which allows to predict the absorption of these chemicals. elements from the tissues surrounding the fungal body, as the main mechanism of their accumulation in concentrations that cause the formation of areas of high radiation density.

Keywords

fungal body, sinusitis, electronic microscopy, masspectrometry, endodontic materials, zinc, calcium.


Reference

  1. Sharif MS, Ali S, Nisar H. Frequency of Granulomatous Invasive Fungal Sinusitis in Patients with Clinical Suspicion of Chronic
    Fungal Rhinosinusitis. Cureus. 2019;11(5):e4757. Published 2019 May 25. doi:10.7759/cureus.4757.
  2. Fanucci E, Nezzo M, Neroni L, Montesani L Jr, Ottria L, Gargari M. Diagnosis and treatment of paranasal sinus fungus ball of odontogenic origin: case report. Oral Implantol (Rome). 2014 Apr 4;6(3):63-6.
  3. Yoon YH, Xu J, Park SK, Heo JH, Kim YM, Rha K-S. A retrospective analysis of 538 sinonasal fungus ball cases treated at a single tertiary medical center in Korea (1996-2015). International Forum of Allergy & Rhinology. 2017 Nov;7(11):1070-
    1075. DOI: 10.1002/alr.22007.
  4. Salamah MA, Alsarraj M, Alsolami N, Hanbazazah K, Alharbi AM, Khalifah WSr. Clinical, Radiological, and Histopathological Patterns of Allergic Fungal Sinusitis: A Single-Center Retrospective Study. Cureus, 2020;12(7), e9233.
    doi.org/10.7759/cureus.9233.
  5. Grosjean P, Weber R. Fungus balls of the paranasal sinuses: a review. Eur Arch Otorhinolaryngol. 2007 May;264(5):461-70. doi: 10.1007/s00405-007-0281-5.
  6. Nicolai P, Mensi M, Marsili F, Piccioni M, Salgarello S, Gilberti E, et al. Maxillary fungus ball: zinc-oxide endodontic materials as a risk factor. Acta Otorhinolaryngol Ital. 2015 Apr;35(2):93-6.
  7. Jiang Z, Zhang K, Huang W, Yuan Q. A Preliminary Study on Sinus Fungus Ball with Micro CT and X-Ray Fluorescence Technique. PLoSONE. 2016;11(3):e0148515. doi:10.1371/journal. pone.0148515.
  8. Montone KT. Pathology of Fungal Rhinosinusitis: A Review. Head and neck pathology. 2016;10(1), 40-6.
    doi: 10.1007/s12105-016-0690-0.
  9. Chakrabarti A, Denning DW, Ferguson BJ, Ponikau J, Buzina W, Kita H, et al. Fungal rhinosinusitis: a categorization and definitional schema addressing current controversies. Laryngoscope. 2009 Sep;119(9):1809-18.
    doi: 10.1002/lary.20520.
  10. Mitin Yu, Krynychko L, Liventsov V, Aksionova N, Krynychko F, Motailo O. [Micetoma vs Aspergillus – who digs a pit for others may fall himself there in.] Suchasna stomatolohiya. 2015;(1):59-61. [Article in Ryssian]. Available from: http://nbuv.gov.ua/UJRN/ss_2015_1_14.
  11. Yamauchi T, Tani A, Yokoyama Sh, Ogawa H. Assessment of noninvasive chronic fungal rhinosinusitis by cone beam CT: comparison with multidetector CT findings. Fukushima J Med Sci. 2017 Aug 9;63(2):100-105. doi: 10.5387/fms.2016-16.
  12. Shkorbotun YaV, Skoryk MA. Features of diagnostics of fungal ball of paranasal sinuses of iatrogenic genesis. Otorhinolaryngology. 2020;(5-6):4-11. DOI 10.37219/2528-8253-2020-6-04.
  13. Grjibovski AM, Ivanov SV. Gorbatova MA. [Descriptive statistics using statistica and spss software]. Science&Healthcare. 2016; 1: [Article in Russian]. URL: https://cyberleninka.ru/article/n/opisatelnaya-statistika-s-ispolzovaniem-pake-
    tov-statisticheskih-programm-statistica-i-spss.
  14. Churin BV. [Biologically active chemical elements in mammalian metabolism]. Mezhdunarodnyiy zhurnal prikladnyih i fundamentalnyih issledovaniy. 2017;(8-2):237-42. doi:10.17513/mjpfi.11792. Available from: http://www.applied-re-
    search.ru/ru/article/view?id=11792. [Article in Russian].
  15. Dindo M, Costanzi E, Pieroni M, Costantini C, Annunziato G, Bruno A, et al. Biochemical Characterization of Aspergillus fumigatus AroH, a Putative Aromatic Amino Acid Aminotransferase. Front. Mol. Biosci. 2018;5:104. doi: 10.3389/
    fmolb.2018.00104.
  16. Todoriko LD, Shevchenko OS, Ovcharenko IA. [Morphological mechanisms of destruction of the connective tissue of the lungs in tuberculosis]. Infusion&Chemotherapy 2019;2:14-20. DOI: 10.32902/2663-0338-2019-2-14-20. [Article in
    Ukrainian].
  17. Kuzyk YuI. [Use of matrix metalloproteinase-9 (MMP-9) and its tissue inhibitor (TIMP-1) in the pathomorphological diagnosis of carotid pathology: literature review and own observations]. Patologiya. 2016;1(36):37-44.
    DOI: 10.14739/2310-1237.2016.1.71935. [Article in Russian].
  18. Naumenko AМ, Krynychko LR, Rezmak M. Treatment of patients with the cycle of the supermontal arm of the ontogenic genesis. Ukrainian scientific medical youth journal. 2019;1(109):18-24. doi.org/10.32345/USMYJ.1(109).2019.18-24.
  19. Cui H, Liu X, Li K, Cao T-T, Cui C, Wang J-Y. Mechanism of Hg (II), Cd (II) and Pb (II) ions sorption from aqueous solutions by Aspergillus niger spores. Separation Science and Technology. 2020;55(5):1-12. https://doi.org/10.1080/01496395
    .2019.1576733.
  20. Xue WN, Peng YB. Study on Environmental Materials with Aspergillusniger as Adsorbent for Sequestering Pb (II) from Aqueous Solution. AMR 2013;676:119-23. https://doi.org/10.4028/www.scientific.net/amr.676.119.
 

© 2019, Public Organization «Ukrainian Scientific Medical Society of Otorhinolaryngologists»